DNA
MHETase

Part:BBa_K4043002:Design

Designed by: Jie Wang   Group: iGEM21_United_Shanghai_HS   (2021-09-22)


MHETase expressed genes in frame


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 648
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 648
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 648
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 330
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 786
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

We deleted the signal peptide sequence before the ISF6_0224 (expressing MHETase) gene fragments, and added the gene sequence of the signal peptide SPLamB before the fusion sequence to increase the exocrine expression of the protein.

References